מעבדות מחקר לקורונה

המידע המובא בעמוד זה יעסוק בהנחיות בטיחות הנוגעות למעבדות מחקר לקורונה

הנחיות בטיחות למעבדות העוסקות בקורונה

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להכניס כקישור על המילים

   BSL

דבי תכין פרוטוקול שימוש בסטריקון

יעילות וסטריליזציה של אוטוקלב

ואסף יצלם תמונה של הפרודקט

  

The Safety Unit – Sackler Faculty of Medicine

 

 

Biosafety Guidelines

Considerations for Handling Potential SARS-Cov-2 Samples in Research Laboratories

 

  1. Introduction

 

Biosafety Level Two Plus (BSL2+) describes laboratories where work with microorganisms is conducted in a BSL-2 laboratory with biosafety practices and procedures typically found at BSL-3.

BSL2+ means that safety practices are enhanced over and above the practices required at BSL-2.

 

Highlights of SARS-Cov-2 laboratory biosafety

  • The use of BSL-3 practices in a BSL-2 laboratory = BSL2+
  • All procedures must be performed based on risk assessment and only by personnel with demonstrated capability in strict observance to any relevant protocols at all times
  • Initial processing (before inactivation) of all specimens should take place in a validated biological safety cabinet (BSC)
  • Non-propagative diagnostic laboratory work (e.g. sequencing, antibodies isolation, RT-PCR) should be conducted at facilities and procedures equivalent to BSL-2+
  • Propagative work (e.g. virus culture, isolation or neutralization assays) should be at a containment laboratory with inward directional airflow (BSL-3). There are no BSL-3 laboratories at Tel Aviv University. The propagative category is forbidden at Tel Aviv University
  • Appropriate disinfectants with proven activity against enveloped viruses should be used (e.g. hypochlorite (bleach), alcohol, hydrogen peroxide
  • Patient specimens from suspected or confirmed cases should be transported as UN3373, “Biological. Substance, Category B”. The packaging consists of 3 components: a primary receptacle; a secondary package; a Rigid outer package
  •  
  • Viral cultures or isolates are forbidden to enter Tel Aviv University

 

 

  1. Laboratory biosafety: conventional clinical and microbiology laboratories

Sackler laboratories (BSL-2+)

  • Initial processing (before inactivation) of all specimens (peripheral blood, serum) should take place in biological safety cabinet (BSC)
  • All technical procedures should be performed in a way that minimizes the generation of aerosols and droplets
  • Centrifugation of blood specimens is in safety cups and sealed rotor near the BSC
  • First barrier: personal protective equipment (PPE)
  • The PI is ultimately responsible for the safety of his/her personnel
  • All work is conducted in BSC, including loading and unloading of centrifuge safety cups for the tabletop centrifuge located within the laboratory

 

  1. The laboratory personnel
  • PPE includes: mask N-95; double gloves; lab coat; disposable lab coat back tide; cover sleeves; face shield; cover shoes
  • Masks should be changed every 3 hours; gloves should be change frequently; face shield should be decontaminated with disinfection; wash the hands frequently; do not touch the face
  • Training and competency verification for each procedure; specific training in handling pathogenic agents
  • Supervision by scientists competent in handling infectious agents
  • Hepatitis B vaccine is required for workers handling PBMC (peripheral blood mononuclear cells), serum and pulmonary lavage. Hepatitis B vaccine includes 3 dose series (0, 1, 6 months scheduled)

 

  1. Biohazard waste
  • The use of two biohazard bags
  • Autoclave must be performed daily, immediately at the end of the work
  • Forbidden to store biohazard waste bags and to transfer them to another Unit
  • The solid non-sharp waste (tips, pipettes) is collected within the BSC in a small biohazard container lid (2/3 full, the bag is closed and removed to the biohazard waste
  • Liquid waste is treated with 1:5 diluted bleach solution, allowed to sit at least 30 minutes, and then carefully disposed of via the sink
  • Sharps, Pasteur pipettes and needles are prohibited in the BSL2+

 

  1. Biological spill kit
  • Kit includes: gloves, PVC gloves, Mask N95 or N99, pads, bleach solution diluted 1:5, tongs, dust pan, goggles

 

 

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