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ארונות לציוד שפך ביולוגי

לפתוח עמוד קורונה:

דבי תשל טקסט

דבי תשלח הקדמה

יש תמונה

 

 

 

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להכניס אינפוגרפיקה

 

                                                            March 16, 2020

The Safety Unit – Sackler Faculty of Medicine

 

Ethidium Bromide (EtBr) handling waste

אסף ישלח תמונות

דבי תשלח טקסט מקדים

Ethidium bromide stock: 1mg/ml; 1mg/ml

One drop of EtBr= 50 ml

EtBr concentration in 100ml agarose gel is 0.5 mg/ml

EtBr concentration in running buffer is 0.1 mg/ml

Ethidium bromide solutions at concentration of less than 1 mg/ml are not considered hazardous waste

 

Table 1. EtBr concentration in gel agarose/running buffer

 

No. drops EtBr

Gel agarose (ml)

EtBr concentration

 (gel or running buffer)

Waste

Observation

1 drop

100 ml

0.5 mg/ml

Regular trash

 

2 drops

100 ml

1 mg/ml

Regular trash

 

3 drops

100 ml

1.5 mg/ml

Chemical waste

Plastic bag for Tabib company

1 drop

50 ml

1 mg/ml

Regular trash

 

2 drops

50 ml

2 mg/ml

Chemical waste

Plastic bag for Tabib company

3 drops

50 ml

3 mg/ml

Chemical waste

Plastic bag for Tabib company

-----

Running buffer

0.1 mg/ml

Regular trash

Drain disposal

 

 

*ציוד והסבר שפף ביולוגי

 

                                          

 

 

 

 

 

 

 

 

 

*רשימת גורמים ביולוגיים

*מנדפים ביולוגים: סוג ותוקף

*פסולת ביולגי: חוקים ואיסף (חברה טביב)

*פסולת ביולגי: אוטוקלב

*משטפות עיניים

אטידיום ברומיד (פסולת)

*פסולת סיטוטוקצית
 

 

 

                                                            March 19, 2020

The Safety Unit – Sackler Faculty of Medicine

 

Biological handling waste

 

 

 

  1. All the biological waste should be disposed in a Biohazard bag for autoclave.
  2. Biological waste includes: plastic/pipetts/tips from tissue culture, bacteria, viruses, parasites, blood, animal or human tissues, fungi.
  3. Sharp instruments in touched with biological material should be disposed in Sharp bin container.
  4. The biohazard and sharp bin container will be autoclaved.
  5. The disinfection autoclave process can be done by the Department (‘in house”) or by an external company (Tabib). Each Department organize its own process. Tabib collects the Biological waste once a week from the Yellow containers.

 

 

 

 

 

 

 

 

Sincerely,

 

 

Dr. Debora Rapaport, PhD                                                           

                                    

Safety Officer – Sackler Faculty of Medicine

 

 

 

הטבלאות להלן הועתקו לדף ייחודי בשם /safty-biology-biosafety-%20level

 

 

BSL-2+           

BSL-2          

BSL-1                

Work practices      

Laboratories in which work with microorganisms are conducted in a BSL-2 laboratory with biosafety procedures typically found at BSL-3.

 

The risk assessment process, may determine that safety practices, over and above those required at BSL-2 needed for a research project, yet a more complex BSL-3 laboratory facility is not necessary.

Agents that pose moderate hazard to personnel and environment.

Agents that are associated with human disease. Treatments are available.

Example: Human or Primate Cells, Blood or any human body fluids, Patient specimens. Herpes Simplex Virus, HIV, Salmonellae, Hepatitis B, Listeria, Infected animals with BSL-2 agents.

Well characterized agents

Agents. Not known/unlikely to cause disease, in healthy human adults, immunocompetent adults, and present minimal hazard potential, to laboratory personnel and to the environment.

Prophylactic treatment available.

Example:  E. coli (K-12 strains), yeast

characterization

restricted

Limit access to lab while BSL-2 work is being conducted

Not recommended

Public access        

Not permitted for biohazardous materials

Permitted only for low risk procedures

Permitted

Bench-top work

 

 

BSL-2+           

BSL-2          

BSL-1                

Work practices      

Required (wraparound disposable preferable)

Required when work with BSL – 2 is being conducted

Recommended

Lab coats

Double bag required**

Double bag required**

Required**

Biohazard bag

Daily; immediately following work with biohazardous materials, and following any spill

Daily and following any spill

Daily and following any spill

Decontamination

Not permitted at any time; Food or drink may not be brought into or through lab.

must not be permitted in

Laboratory areas. Food must be stored outside the laboratory area in cabinets or refrigerators designated and used for this purpose

must not be permitted in

Laboratory areas. Food must be stored outside the laboratory area in cabinets or refrigerators designated and used for this purpose

 

 

Eating, drinking applying lip balm, etc.

Required (wraparound disposable preferable)

Required when work with BSL – 2 is being conducted

Recommended

Lab coats

 

 

    

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