ד"ר תמר גיגר

Education

2002-2007- The Hebrew University of Jerusalem, Ph.D. in the Department of Biological Chemistry.

2000-2002- The Hebrew University of Jerusalem, M.Sc . in the Department of Biological Chemistry, with Distinction.

 1997-2000- The Hebrew University of Jerusalem, B.Sc . in Life Sciences, with Distinction.

Academic Positions

2008-2011- Post doctoral research fellow, the laboratory of Prof. Matthias Mann, department of Proteomics and Signal Transduction, the Max Planck Institute of Biochemistry, Martinsried, Germany.

2011- Tenure track position (senior lecturer) at the Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv.

המעבדה עוסקת במחקר פרוטאומי של סרטן השד. אנו משתמשים בשיטות של מס-ספקטרומטריה מתקדמת  לבחינה של שינויים בחלבונים ובמודיפיקציות שלהם בצורה גלובאלית, ואנליזה של דוגמאות קליניות של סרטן השד. המעבדה שמה דגש על שינויים מטאבוליים המתרחשים בתאים הסרטניים, ומשתמשת במידע הכולל על הפרוטאום להבנת הרשתות המטאבוליות. שילוב של המחקר הפרוטאומי עם שיטות ביוכימיות וגנטיות יגלה את החשיבות של חלבוני מפתח להתפתחות הגידולים ויבחן את הפוטנציאל שלהם כמטרות תראפויטיות ודיאגנוסטיות. 

1.           Reuveni, H., Geiger, T., Geiger, B. & Levitzki, A. Reversal of the Ras-induced transformed phenotype by HR12, a novel ras farnesylation inhibitor, is mediated by the Mek/Erk pathway. J Cell Biol 151, 1179-1192 (2000).

2.           Reuveni, H., Livnah, N., Geiger, T., Klein, S., Ohne, O., Cohen, I., Benhar, M., Gellerman, G. & Levitzki, A. Toward a PKB inhibitor: modification of a selective PKA inhibitor by rational design. Biochemistry 41, 10304-10314 (2002).

3.           Klein, S., Geiger, T., Linchevski, I., Lebendiker, M., Itkin, A., Assayag, K. & Levitzki, A. Expression and purification of active PKB kinase from Escherichia coli. Protein Expr Purif 41, 162-169 (2005).

4.           Geiger, T. & Levitzki, A. Loss of robustness and addiction to IGF1 during early keratinocyte transformation by human Papilloma virus 16. PLoS One 2, e605 (2007).

5.           Geiger, T., Sabanay, H., Kravchenko-Balasha, N., Geiger, B. & Levitzki, A. Anomalous features of EMT during keratinocyte transformation. PLoS One 3, e1574 (2008).

6.           Zanivan, S., Gnad, F., Wickstrom, S.A., Geiger, T., Macek, B., Cox, J., Fassler, R. & Mann, M. Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-5326 (2008).

7.           Geiger, T., Cox, J. & Mann, M. Proteomic changes resulting from gene copy number variations in cancer cells. PLoS Genet 6 (2010).

8.           Geiger, T., Cox, J. & Mann, M. Proteomics on an Orbitrap benchtop mass spectrometer using all-ion fragmentation. Mol Cell Proteomics 9, 2252-2261 (2010).

9.           Geiger, T., Cox, J., Ostasiewicz, P., Wisniewski, J.R. & Mann, M. Super-SILAC mix for quantitative proteomics of human tumor tissue. Nat Methods 7, 383-385 (2010).

10.       Geiger, T. & Geiger, B. Towards elucidation of functional molecular signatures of the adhesive-migratory phenotype of malignant cells. Semin Cancer Biol 20, 146-152 (2010).

11.       Lundberg, E., Fagerberg, L., Matic, I., Geiger, T., Cox, J., Klevebring, D., Älgenäs, C., Lundeberg, J., Mann, M & Uhlen, M. Highly ubiquitous expression of the transcriptome and proteome in three functionally different human cell lines. Mol Syst Biol 6, 450 (2010).

12.       Geiger, T., Wisniewski, JR., Cox, J., Zanivan, S., Kruger, M., Ishihama, Y. & Mann, M. Use of stable isotope labeling by amino acids in cell culture (SILAC) as an internal standard in quantitative proteomics. Nat Protocols 6, 147-157 (2011).

13.       Thakur, S.*, Geiger, T.*, Chatterjee, B., Bandilla, P., Fröhlich, F., Cox, J. & Mann, M. Deep proteome coverage in single-run liquid chromatography tandem mass spectrometry.  Mol Cell Proteomics. 2011 May 17 [Epub ahead of print] * Equal contribution. 

14.       Meves, A., Geiger, T., Zanivan, S., DiGiovanni, J., Mann, M. & Fässler, R. β1 integrin cytoplasmic tyrosines promote tumorigenesis in a phosphorylation-independent manner. Proc Natl Acad Sci USA.

15.       Nagaraj, N., Wisniewski, J.R., Geiger, T., Cox, J., Kircher, M., Kelso, J., Paabo, S. & Mann, M. Ultra-deep proteome and transcriptome mapping of a human cancer cell line. Mol Syst Biol 7, 548 (2011).

16.    Geiger, T., Wehner, A., Schaab, C., Cox, J., & Mann, M. Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins. Mol Cell Proteomics.  2012 Jan 25. [Epub ahead of print].

17.    Schaab, C., Geiger, T., Stoehr, G., Cox, J., & Mann, M. Analysis of high-accuracy, quantitative proteomics data in the MaxQB database. Mol Cell Proteomics. 2012 Feb 2. [Epub ahead of print].