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Introduction: Biosafety level (Table)

Biosafety level designations are based on a composite of the design features, construction, containment facilities, equipment, practices and operational procedures required for working with agents from the various microorganisms risk groups.

Laboratory facilities are designated as:

Biosafety Level 1: basic

Biosafety Level 2: basic

Biosafety Level 2+: BSL-2 laboratory with biosafety practices and procedures typically found at BSL-3

Biosafety Level 3: containment, as Level 2 plus special clothing, controlled access, directional airflow

Biosafety Level 4: maximum containment. As Level 3 plus airlock entry, shower exit, special waste disposal

BSL-2+	BSL-2	BSL-1	Work practices
*Laboratories in which work with microorganisms are conducted in a BSL-2 laboratory with biosafety procedures typically found at BSL-3.* *The risk assessment process, may determine that safety practices, over and above those required at BSL-2 needed for a research project, yet a more complex BSL-3 laboratory facility is not necessary.*	*Agents that pose moderate hazard to personnel and environment.* *Agents that are associated with human disease. Treatments are available.* *Example: Human or Primate Cells, Blood or any human body fluids, Patient specimens. Herpes Simplex Virus, HIV, Salmonellae, Hepatitis B, Listeria, Infected animals with BSL-2 agents.*	*Well characterized agents* *Agents. Not known/unlikely to cause disease, in healthy human adults, immunocompetent adults, and present minimal hazard potential, to laboratory personnel and to the environment. Prophylactic treatment available.* *Example: E. coli (K-12 strains), yeast*	*Characterization*
restricted	Limit access to lab while BSL-2 work is being conducted	Not recommended	Public access
Not permitted for biohazardous materials	Permitted only for low risk procedures	Permitted	Bench-top work
			Lab coats
			Biohazard bag

לאחד טבלאות

BSL-2+	BSL-2	BSL-1	Work practices
Required (wraparound disposable preferable)	Required when work with BSL – 2 is being conducted	Recommended	Lab coats
Double bag required**	Double bag required**	Required**	Biohazard bag
Daily; immediately following work with biohazardous materials, and following any spill	Daily and following any spill	Daily and following any spill	Decontamination
Not permitted at any time; Food or drink may not be brought into or through lab.	must not be permitted in Laboratory areas. Food must be stored outside the laboratory area in cabinets or refrigerators designated and used for this purpose	must not be permitted in Laboratory areas. Food must be stored outside the laboratory area in cabinets or refrigerators designated and used for this purpose	Eating, drinking applying lip balm, etc.
Required (wraparound disposable preferable)	Required when work with BSL – 2 is being conducted	Recommended	Lab coats